recombinant human pro ngf cleavage resistant (Alomone Labs)

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Alomone Labs recombinant human pro ngf cleavage resistant
Recombinant Human Pro Ngf Cleavage Resistant, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pro+ngf/Recombinant+human+proNGF+(cleavage+resistant)+protein/pm37579565-61-14-19
Average 91 stars, based on 8 article reviews

recombinant human pro ngf cleavage resistant - by Bioz Stars, 2026-09

91/100 stars

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Injection:

Article Title: The p75 NTR SIGNALING CASCADE MEDIATES MECHANICAL HYPERALGESIA INDUCED BY NERVE GROWTH FACTOR INJECTED INTO THE RAT HIND PAW
Article Snippet: .. A non-hydrolyzable mutated homologue of Pro-NGF, mPro-NGF (Alomone Labs, Jerusalem, Israel) was reconstituted in PBS, then centrifuged at 10,000×g for 5 min to remove any undissolved particulates before paw injection. .. C2-ceramide (Enzo Life Sciences, Farmingdale, NY) was dissolved initially as a 25 μg/μL DMSO stock solution, stored at −80°C in aliquots, then diluted to 20 μg/10 μL DMSO before the injection.

Blocking Assay:

Article Title: Inhibitors of p75 receptor and their uses
Article Snippet: .. The procedure immobilized 50 ng/well NGF, or pro-NGF (Alomone Labs, Israel) onto polystyrene 96-well microtest plates, with washing and blocking with binding buffer (BB; phosphate buffered saline containing 0.5% bovine serum albumin). .. The following primary reagents in BB were added: recombinant chimeric p75-Fc or recombinant chimeric TrkA-Fc (R&D), or anti-NGF mAb NGF30 (Saragovi et al., 1998); each at 50 nM.

Binding Assay:

Saline:

Article Title: Fluoxetine Prevents Oligodendrocyte Cell Death by Inhibiting Microglia Activation after Spinal Cord Injury
Article Snippet: Protein sample (50 μg) was separated on SDS/polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Millipore). .. Membranes were blocked with 5% nonfat skim milk or 5% bovine serum albumin in Tris-buffered saline solution with 0.1% Tween 20 for 1 h at room temperature and then incubated with primary Abs against p-p38-MAPK (1:1000; Cell Signaling Technology), p38-MAPK (1:1000; Cell Signaling Technology), pro-NGF (1:1000; Alomone Labs, Jerusalem, Israel), p-c-Jun (1:1000; Cell signaling Technology), c-Jun (1:500; Santa Cruz Biotechnology, Santa Cruz, CA), cleaved caspase-3 (1:1000; Cell Signaling Technology), MBP (1:500; Millipore), and RhoA (1:1000; Santa Cruz Biotechnology) overnight at 4°C. .. Membranes were then processed with horseradish-peroxidase–conjugated secondary Ab (Jackson ImmunoResearch, West Grove, PA).

Mutagenesis:

Article Title: p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3
Article Snippet: Cells were transfected with 2–4 μg of expression plasmids using transfectin or FuGENE reagents (Bio-Rad, Espoo, Finland). .. Cells were stimulated with different concentrations of NGF or pro-NGF (cleavage-resistant, mutant protein) (Alamone Labs) for various periods of times or with 1 μ m simvastatin (Sigma). .. In some experiments, the broad range caspase inhibitor Boc-aspartyl (Ome)-fluoromethyl ketone (Boc; Calbiochem), the caspase-3 inhibitor Z-DEVD-fmk (BD Biosciences), and the SREBP1 site 1 protease (S1P) inhibitor ( 23 ), PF-429242 (Tocris Bioscience, Bristol, UK), were used.

Cell Culture:

Article Title: Renin-Angiotensin-Aldosterone System Inhibitors Prevent the Onset of Oxaliplatin-Induced Peripheral Neuropathy: A Retrospective Multicenter Study and in Vitro Evaluation.
Article Snippet: Undifferentiated cells were cultured in RPMI 1640 medium (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% horse serum (Life Technologies NZ Ltd., Auckland, New Zealand), 5% fetal bovine serum (Life Technologies Corporation, Grand Island, NY, U.S.A.), and 1% penicillinstreptomycin (Nacalai Tesque), under 95% air and 5% CO2 at 37 °C. .. PC12 cells (1 × 103 cells/well) were seeded in 96-well plates and cultured for 24 h; then, the serum-free medium was replaced, supplemented with 100 ng/mL pro-NGF (nerve growth factor; Alomone Labs, Jerusalem, Israel). .. After 48 h of pro-NGF treatment, differentiated cells exhibiting an increased neurite outgrowth from cell bodies were cultured in serum-free medium containing 0.1, 1, and 10 μM OXA (Wako Pure Chemical Corporation, Osaka, Japan), 1 and 10 μM RAASIs with candesartan cilexetil, enalapril maleate, aliskiren hemifumarate (Wako Pure Chemical Corporation), and spironolactone (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 10 and 100 μM duloxetine hydrochloride (Tokyo Chemical), 1 μM SCH 772984 (Cayman Chemical Company, Ann Arbor, MI, U.S.A.), or 500 μM SQ 22536 (Tokyo Chemical) for 2 d. Changes in the length of neurites were obtained with a phase-contrast microscope (CKX41, Olympus Corporation, Tokyo, Japan) equipped with a digital camera (Olympus E330-ADU12X) and expressed as the ratio to that of the control group.

Incubation:

Article Title: Serum BDNF and NGF Modulation by Olive Polyphenols in Alcoholics during Withdrawal
Article Snippet: Many studies have suggested possible relationships between the neurotrophins brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) and alcohol addiction.. Previous reports demonstrated severe changes in these neurotrophins in the serum of alcohol dependent patients and during withdrawal.. Alcohol dependence syndromes during consumption and/or withdrawal are also characterized by elevated oxidative stress.